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1. Immunomodulatory Effects Of Flumequine And Enrofloxacin On Newcastle Disease Virus Vaccinated

by Waseem Abbas | Prof. Dr. Muhammad Akram Muneer | Dr. Mansur-ud-Din Ahmad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2005Dissertation note: An experiment was conducted to determine whether Flumequine and Enrofloxacin supplementation has any immunomodulatory effects on broiler chicks. A total of 192 one day old broiler chicks were randomly divided into four groups, each consisting of 48 chicks. Each group was further divided into 2 subgroups of 24 chicks. The chicks in group 1st were administered Flumequine, those in group 2 were treated with, Enrofloxacin and those in group 3 were treated with cyclophosphamide. Chicks in group 4 were not given any treatment. The parameters of investigation included the effects of Flumequine and Enrofloxacin treatment on live weight gain, feed conversion ratio, effect on various lymphoid organs (bursa of Fabricius, thymus, spleen and liver) and immune response of treated chicks to NDV-vaccination, post field NDV challenge mortality. Data presented in this study indicated that the Flumequine treated chicks had higher mean body weights, better FCR, higher NDV HI antibody, lesser overall mortality, no NDV post challenge mortality and no detrimental effects on their lymphoid organs, compared to the cyclophosphamide treated, and untreated chicks. The overall findings of this study clearly demonstrate that the use of Flumequine has good effect on growth and performance of the treated chickens. (Key words: Flumequine, Enrofloxacin, Immunomodulation, Broiler) Availability: Items available for loan: UVAS Library [Call number: 0881,T] (1).

2. An Epidemiological Study Of Nosocomial Infections At Mayo Hospital, Lahore

by Tayyaba Ijaz (Phd) | Prof. Dr. Muhammad Akram Muneer | Dr. Mansur-ud-Din Ahmad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 2005Dissertation note: The present study was designed to investigate the Prevalence of Etiological Agents of Nosocomial Infections in Mayo Hospital, Lahore-Pakistan of the 32,620 patients studied during 1997-2001; a total of 4502 (13.80%) patients acquired various types of nosocomial infections during their stay at Hospital. Clinical samples collected from various types of patients consisted of 1040 samples of Pus & Wound Swabs, 109 samples of blood; 115 of Pleural Fluids, 286 of Ascetic Fluids, 37 of Cerebrospinal Fluid, 1398 of Urine, 988 of Sputum; 329 of Burn Swabs, 99 of Patient Body Devices and 101 of Fecal and Drainage Material. The routine techniques for isolation. Identification through Biochemical, Serological and Antibiotic Sensitivity Testing were used for studying the Bacteriology of the selected samples. The present findings revealed that from a total of 4502 samples, 1287 Strains of Staphylococci, 429 Strains of Streptococci, 328 Strains of Enterococci, 781 Strains of Pseudomonas, 349 Strains of Enterobacter, 41 Strains of Acinetobacter, 26 Strains of Klebsiella, 140 Strains of Proteus, 1031 Strains of Escherichia, 67 Strains of Serratia, 93 Strains of Haemophilus, 119 Strains of other types of Gram Positive Bacteria, 13 Strains of other types of Gram Negative Bacteria, and 189 Strains of Yeast and Fungi were found as Etiological Agent for Nosocomial Infections. Availability: Items available for loan: UVAS Library [Call number: 0912,T] (1).

3. Immmunobiolotical Observations On Avian Influenza Virus Types H7 And H,

by Shahid Iqbal | Prof. Dr. Muhammad Akram Muneer | Dr. Mansur-ud-Din Ahmad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2007Dissertation note: The present study was designed to 'find the prevalence of Avian Influenza disease in and around Lahore in commercial and household poultry. A total of 1000 blood and 500 cloacal swabs were collected from Broilers, Broiler-Breeders, Ducks, Pigeons, Sparrows, Quails and Desi Chickens. The blood samples from all the flocks showed non-significant titers while vaccinated flocks showed protective titers. All the cloacal swabs were negative for virus isolation. The final conclusions from this study were the following.i.e. - Avian influenza caused by H7 & H9 type is not prevalent in broiler and broiler breeders in and around Lahore. - The vaccinated poultry flocks showed higher titers of antibodies as compared to non-vaccinated flocks which means that vaccine can play a vital role in protection of bird from H7 & H9. Availability: Items available for loan: UVAS Library [Call number: 0963,T] (1).

4. Factors Affecting Hemagglutination Potential Of Avain Influenza Viuruses (H5, H7, H9 Subtypes)

by Mubashir Hussain | Prof. Dr. Muhammad Akram Muneer | Dr. Mansur-ud-Din Ahmad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 2007Dissertation note: The objective of this study was to standardize hemagglutination and hemagglutination inhibition tests for AIV H5, H7 and H9 subtypes. These subtypes were propagated in 09-day old chicken embryonated eggs and after 72 hours post incubation the allantoic fluid (AF) was harvested and confirmed by spot agglutination test and by AGPT. While standardizing HA test maximum titers were recorded using 1% RBCs of chicken, human blood group Qe and dog using phosphate buffer saline (PBS) as a diluting agent for washing suspension of erythrocyte and by incubating the micro titer plates at 22c or 37C for 30 minutes or 40 minutes time period. The AIV subtypes eluted rapidly with increase in temperature with maximum elution observed within the time period of 8 hours. The live AIV provided much higher HA titer when compared with the titers obtained from AJV subtypes inactivated with formalin or Binary ethylene imine (BET). The BET was found to have little effect on HA activity as compared to formalin. While standardizing the HI test the best titers were obtained using 4 HA units of AIV antigen as compared to 1 HA and 8 HA units of antigen and by incubating the micro titer plates for 60 minutes period (time given for antigen-antibody reaction before the addition of erythrocytes suspension). Availability: Items available for loan: UVAS Library [Call number: 0984,T] (1).



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